Identification of Bioactive Molecules from Thai Centipede, Scolopendra subspinipes dehaani, Venom

Abstract

Centipede venoms are rich sources of bioactive molecules with various actions. These bioactive molecules obtained from Thai centipede, S. subspinipes dehaani, venom have been investigated by proteomic approach and bioactivity assay. Protein profile analysis by SDS-PAGE technique showed 7 major bands with a molecular weight ranging under 66 kDa. After resolved in 2D-PAGE gel, numerous spots of proteins were found in centipede venom. The trains of major spots were observed at 22 and 45 kDa. The 10 major protein spots were analyzed byliquid chromatography coupled to tandem mass spectrometry(LC-MS/MS). The presence of protease and phospholipase activities in centipede venom was tested by zymographic method andon activity indicator plate (PCY plate), respectively.These molecules were classified into 4 groups including cysteine-rich venom protein, protease, hyaluronidase and phospholipase.