Cultivation of Amanita princeps and Gyrodon suthepensis for Mycorrhizations with Castanopsis acuminatissima and their effects on the host plants

Abstract

© 2019 Friends Science Publishers. The aim of this research was to investigate optimal culture conditions of Amanita princeps and Gyrodon suthepensis for inoculum production of the edible ectomycorrhizal fungi (ECM), and to evaluate the effects of mycelial inoculation of the ECM fungi on mycorrhizations with seedlings of Castanopsis acuminatissima (Blume) A.DC. and the growth of the host plants. Mycelia of A. princeps and G. suthepensis were isolated from basidiocarps of the ECM mushrooms in Northern Thailand. Mycelia of A. princeps grew well on potato dextrose agar (PDA) and modified Melin Norgans (MMN) agar at pH 6 and at 28°C in the dark condition. Mycelia of G. suthepensis grew well on PDA and Pachlewski (PACH) agar supplemented with 1.0 g L -1 yeast extract at 25°C and at pH 6 and 7. The dark condition or the daily light and dark condition did not have significant effect on mycelial growth of G. suthepensis. ECM roots of C. acuminatissima inoculated with A. princeps possessed thick mantle hyphae and formed cortical Hartig net, while ECM roots of the plants inoculated with G. suthepensis possessed thin mantle hyphae and formed epidermal Hartig net. The ECM fungi significantly increased survival and growth of the host plants. G. suthepensis was more effective in increasing the growth of C. acuminatissima seedlings than A. princeps. The results of this research can be used for production of ECM host seedlings for forest restoration.