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dc.contributor.authorNaruemon Rakchaien_US
dc.contributor.authorAran H-Kittikunen_US
dc.contributor.authorWolfgang Zimmermannen_US
dc.date.accessioned2019-05-07T09:59:45Z-
dc.date.available2019-05-07T09:59:45Z-
dc.date.issued2018en_US
dc.identifier.issn0125-2526en_US
dc.identifier.urihttp://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8956en_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/64069-
dc.description.abstractThe production of whole-cell lipase by Aspergillus nomius ST57 for the synthesis of biodiesel (fatty acid methyl esters; FAME) from palm oil was investigated. The whole-cell lipase produced by A. nomius ST57 catalyzed both transesterification and esterification reactions. The whole-cell lipase production in a shake-flask culture was optimized by using Taguchi experimental design and response surface methodology. The optimal culture medium was composed of olive oil 22.91 g/L, yeast extract 10.52 g/L, (NH4)2SO4 1.07 g/L and KH2PO4 1 g/L with the initial pH 5.0. The optimal conditions were 1 mL of 5´106 spores/mL inoculum in 50 mL of medium at 150 rpm and 30 °C for 48 h. The hydrolytic and transesterification activities of the whole-cell lipase were 2 times and 3 times higher than in the non-optimized medium and conditions. The optimal conditions for FAME synthesis by the whole-cell lipase of A. nomius ST57 consisted of 25 mg dry cell weight, palm oil:methanol 1:3 mol/mol and 15% of 50 mM Tris-HCl buffer pH 7 at 37 °C. After 5 h of reaction, 93.54% FAME was obtained in one step addition of methanol.en_US
dc.languageEngen_US
dc.publisherScience Faculty of Chiang Mai Universityen_US
dc.titleProduction of Whole-cell Lipase from Aspergillus nomius ST57 and Optimization of Methyl Esters Synthesis from Palm Oil in One Stepen_US
dc.typeบทความวารสารen_US
article.title.sourcetitleChiang Mai Journal of Scienceen_US
article.volume45en_US
article.stream.affiliationsDepartment of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90112, Thailand.en_US
article.stream.affiliationsDepartment of Microbiology and Bioprocess Technology, Institute of Biochemistry, University of Leipzig, Leipzig 04103, Germanyen_US
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