Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorNopprarat Tongmuangen_US
dc.contributor.authorUmpa Yasamuten_US
dc.contributor.authorPucharee Songprakhonen_US
dc.contributor.authorThanyaporn Dechtawewaten_US
dc.contributor.authorShilu Malakaren_US
dc.contributor.authorSansanee Noisakranen_US
dc.contributor.authorPa thai Yenchitsomanusen_US
dc.contributor.authorThawornchai Limjindapornen_US
dc.description.abstract© 2018 Elsevier B.V. Dengue hemorrhagic fever is a life-threatening disease caused by the dengue virus (DENV). After DENV enters into host cells, it replicates to generate viral particles to infect other cells. DENV exploits components of the cellular trafficking pathway to achieve effective virion production. Understanding of the proteins required for this trafficking process is essential for revealing the pathogenesis of DENV infection. Coat protein complex and soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), two host protein families in the cellular trafficking pathway, were investigated to elucidate their respective roles during DENV infection. Coat proteins (COPI and COPII) and SNAREs (STX 5 and NSF) were knocked down in a DENV-infected Huh7 cells by RNA interference. Depletion of COPI and COPII, but not of STX5 and NSF, decreased DENV production in DENV-infected Huh7 cells. DENV proteins, including DENV C, prM, E, and NS1, were significantly reduced in COPI-silenced DENV-infected Huh7 cells, when compared to those of control cells. COPI also facilitated DENV production in an endothelial cell line and in all DENV serotypes, indicating the importance of coat protein complex in facilitating DENV infection.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleCoat protein complex I facilitates dengue virus productionen_US
article.title.sourcetitleVirus Researchen_US
article.volume250en_US Universityen_US Mai Universityen_US National Center for Genetic Engineering and Biotechnologyen_US
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.