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dc.contributor.authorThaneeya Chetiyanukornkulen_US
dc.contributor.authorAkira Toribaen_US
dc.contributor.authorTakayuki Kamedaen_US
dc.contributor.authorNing Tangen_US
dc.contributor.authorKazuichi Hayakawaen_US
dc.description.abstractA method is presented for determining monohydroxy polycyclic aromatic hydrocarbons (OHPAHs) having 2-, 3- and 4-rings in human urine by using high-performance liquid chromatography with fluorescence detection. A urine sample containing conjugates of OHPAHs was hydrolysed in the presence of β-glucuronidase/aryl sulfatase and the solution was cleaned up with a solid-phase extraction (C18and silica). Eight OHPAHs, namely 1- and 2-hydroxynaphthalenes, 2-hydroxyfluorene, 2-, 3- and 4-hydroxyphenanthrenes, 3-hydroxyfluoranthene and 1-hydroxypyrene, were separated and 1- and 9-hydroxyphenanthrenes co-eluted on an alkylamide-type reversed-phase column with fluorimetric detection. The urinary concentrations of OHPAHs were quantified by using deuterated 1-hydoxypyrene as an internal standard. The method showed good repeatability for inter- and intra-day precisions as well as good linearity of calibration curves (r2ranged from 0.996 to 0.999). The limits of detection (S/N=3) were in the range from 2.3 fmol to 2.2 pmol per injection. This method was successfully applied to urine samples from non-smoking taxi drivers, traffic policemen and rural villagers of Chiang Mai, Thailand. The results showed higher urinary concentrations of OHPAHs in rural villagers, consistent with higher respiratory exposure to PAHs. © Springer-Verlag 2006.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleSimultaneous determination of urinary hydroxylated metabolites of naphthalene, fluorene, phenanthrene, fluoranthene and pyrene as multiple biomarkers of exposure to polycyclic aromatic hydrocarbonsen_US
article.title.sourcetitleAnalytical and Bioanalytical Chemistryen_US
article.volume386en_US Mai Universityen_US Universityen_US
Appears in Collections:CMUL: Journal Articles

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