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dc.contributor.authorDuriya Fongmoonen_US
dc.contributor.authorAjaya Kumar Shettyen_US
dc.contributor.authorShuhei Yamadaen_US
dc.contributor.authorMakiko Sugiuraen_US
dc.contributor.authorPrachya Kongtawelerten_US
dc.contributor.authorKazuyuki Sugaharaen_US
dc.description.abstractChondroitin sulfate K (CS-K) from king crab cartilage rich in rare 3-O-sulfated glucuronic acid (GlcUA(3S)) displayed neuritogenic activity and affinity toward various growth factors like CS-E from squid cartilage. CS-K-mediated neuritogenesis of mouse hippocampal neurons in culture was abolished by digestion with chondroitinase (CSase) ABC, indicating the possible involvement of GlcUA(3S). However, identification of GlcUA(3S) in CS chains by conventional high performance liquid chromatography has been hampered by its CSase ABC-mediated degradation. To investigate the degradation process, an authentic CS-E tetrasaccharide, Δ4,5HexUA-GalNAc(4S)-GlcUA(3S)- GalNAc(4S), was digested with CSase ABC, and the end product was identified as GalNAc(4S) by electrospray ionization mass spectrometry (ESI-MS). Putative GalNAc(6S) and GalNAc(4S,6S), derived presumably from GlcUA(3S)-GalNAc(6S) and GlcUA(3S)-GalNAc(4S,6S), respectively, were also detected by ESI-MS in the CSase ABC digest of a CS-E oligosaccharide fraction resistant to CSases AC-I and AC-II. Intermediates during the CSase ABC-mediated degradation of Δ4,5HexUA(3S)-GalNAc(4S) to GalNAc(4S) were identified through ESI-MS of a partial CSase ABC digest of a CS-K tetrasaccharide, GlcUA(3S)-GalNAc(4S)-GlcUA(3S)-GalNAc(4S), and the conceivable mechanism behind the degradation of the GlcUA(3S) moiety was elucidated. Although a fucose branch was also identified in CS-K, defucosylated CS-K exhibited greater neuritogenic activity than the native CS-K, excluding the possibility of the involvement of fucose in the activity. Rather, (3S)-containing disaccharides are likely involved. These findings will enable us to detect GlcUA(3S)-containing disaccharides in CS chains to better understand CS-mediated biological processes. © 2007 by The American Society for Biochemistry and Molecular Biology, Inc.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleChondroitinase-mediated degradation of rare 3-O-sulfated glucuronic acid in functional oversulfated chondroitin sulfate K and Een_US
article.title.sourcetitleJournal of Biological Chemistryen_US
article.volume282en_US Pharmaceutical Universityen_US Universityen_US Mai Universityen_US Ministry of Public Healthen_US Science and Technology Agencyen_US
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