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dc.contributor.authorSakorn Pornpraserten_US
dc.contributor.authorKanyakan Sukunthamalaen_US
dc.contributor.authorJittraporn Sacomeen_US
dc.contributor.authorArunee Phusuaen_US
dc.contributor.authorRattika Saetungen_US
dc.contributor.authorTorpong Sanguansermsrien_US
dc.contributor.authorPranee Leechanachaien_US
dc.description.abstractWithout gel electrophoresis and specific probes, the two tubes real-time SYBR-polymerase chain reaction (SYBR-PCR) was setup by using different primer sets: P1/P2 for the detection of wild type α-globin gene alleles and P1/P3 for detection of the allele bearing the Southeast Asian (SEA) type (- -SEA) deletion. Analyses of the cycle threshold (CT) values obtained by each primer set together with a delta-cycle threshold (ΔCT) and CT ratio, showed that lower CT values generated by primer sets P1/P2 and P1/P3 were observed in normal and Hb Bart's hydrops fetalis subjects, respectively. In heterozygous subjects the CT values generated by both sets of primers were similar to each other. There was no overlapping of ΔCT and CT ratio between normal, heterozygous and Hb Bart's hydrops fetalis subjects. Therefore, the two tubes real-time SYBR-PCR could represent a rapid, cost effective, high-throughput assay for screening of carriers and prenatal diagnosis of α-thalassemia-1 (α-thal-1) with the SEA type (- -SEA) deletion. Copyright © Informa Healthcare USA, Inc.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleAnalysis of real-time SYBR-polymerase chain reaction cycle threshold for diagnosis of the α-thalassemia-1 Southeast Asian type deletion: Application to carrier screening and prenatal diagnosis of Hb Bart's hydrops fetalisen_US
article.volume32en_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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