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dc.contributor.authorArthitaya Kawee-aien_US
dc.contributor.authorNuntinee Ritthibuten_US
dc.contributor.authorApisit Manassaen_US
dc.contributor.authorChurairat Moukamnerden_US
dc.contributor.authorThunnop Laokuldiloken_US
dc.contributor.authorSuthat Surawangen_US
dc.contributor.authorSutee Wangtueaien_US
dc.contributor.authorYuthana Phimolsiripolen_US
dc.contributor.authorJoe M. Regensteinen_US
dc.contributor.authorPhisit Seesuriyachanen_US
dc.description.abstract© 2018 Taylor & Francis. Prebiotic substances are extracted from various plant materials or enzymatic hydrolysis of different substrates. The production of fructo-oligosaccharide (FOS) and inulo-oligosaccharide (IOS) was performed by applying two substrates, sucrose and inulin; oligosaccharide yields were maximized using central composite design to evaluate the parameters influencing oligosaccharide production. Inulin from Jerusalem artichoke (5–15% w/v), sucrose (50–70% w/v), and inulinase from Aspergillus niger (2–7 U/g) were used as variable parameters for optimization. Based on our results, the application of sucrose and inulin as co-substrates for oligosaccharide production through inulinase hydrolysis and synthesis is viable in comparative to a method using a single substrate. Maximum yields (674.82 mg/g substrate) were obtained with 5.95% of inulin, 59.87% of sucrose, and 5.68 U/g of inulinase, with an incubation period of 9 hr. The use of sucrose and inulin as co-substrates in the reaction simultaneously produced FOS and IOS from sucrose and inulin. Total conversion yield was approximately 67%. Our results support the high value-added production of oligosaccharides using Jerusalem artichoke, which is generally used as a substrate in prebiotics and/or bioethanol production.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleOptimization of simultaneously enzymatic fructo- and inulo-oligosaccharide production using co-substrates of sucrose and inulin from Jerusalem artichokeen_US
article.title.sourcetitlePreparative Biochemistry and Biotechnologyen_US
article.volume48en_US Mai Universityen_US Universityen_US
Appears in Collections:CMUL: Journal Articles

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