Amycolatopsis oliviviridis sp. Nov., a novel polylactic acidbioplastic-degrading actinomycete isolated from paddy soil

Abstract

© 2018 IUMS. A novel bioplastic-degrading actinomycete, strain SCM_MK2-4T, was isolated from paddy soil in Thailand. The 16S rRNA gene sequence showed that strain SCM_MK2-4Tbelonged to the genus Amycolatopsis, with the highest sequence similarity to Amycolatopsis azurea JCM 3275T (99.4;%), and was phylogenetically clustered with this strain along with Amycolatopsis lurida JCM 3141T(99.3;%), A. japonica DSM 44213T(99.2;%), A. decaplanina DSM 44594T(99.0;%), A. roodepoortensis M29T (98.9;%), A. keratiniphila subsp. nogabecina DSM 44586T(98.8;%), A. keratiniphila subsp. keratiniphila DSM 44409T(98.5;%), A. orientalis DSM 40040T(98.4;%) and A. regifaucium GY080T (98.3;%). A combination of DNA-DNA hybridization results ranging from 42.8±3.2 to 66.2±1.4;% with the type strains of A. azurea and A. lurida and some different phenotypic characteristics indicated that the strain could be distinguished from its closest phylogenetic neighbours. Whole-cell hydrolysates of the strain were shown to contain meso-diaminopimelic acid, arabinose, galactose, glucose, ribose, mannose, rhamnose and xylose. The predominant menaquinone was MK-9(H4). The major cellular fatty acid profile consisted of iso-C15:0, iso-C16:0, summed feature 3 (C16:1ω7c and/or iso-C15:02OH) and C16:0. The polar lipid composition of the strain consisted of phosphatidyl-N-methylethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, aminophospholipids, an unidentified phospholipid and two unidentified glycolipids. The G+C content of the genomic DNA was 68.2 mol%. On the basis of phylogenetic analyses, DNA-DNA hybridization experimentation and the phenotypic characteristics, it was concluded that strain SCM_MK2-4Trepresents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis oliviviridis sp. nov. is proposed. The type strain is SCM_MK2-4T(=TBRC 7186T=JCM 32134T).