Lactobacillus-fermented plant juice as a potential ingredient in cosmetics: Formulation and assessment of natural mouthwash

Abstract

© 2016 The Authors. Published by Innovare Academic Sciences Pvt Ltd. Objective: Oral care cosmetics are essential for all populations and are systematically used to treat oral problems. The chemicals free natural cosmetics are the choice of many people. Thus, this study was aimed to formulate and to assess the natural mouthwash (MW) solution from Lactobacillus-fermented Thai medicinal plants juice. Methods: The selected (betel, green tea, clove, black galingale, mangosteen, and noni) plant juices were subjected to Lactobacillus plantarum mediated fermentation. The fermented plant juices (FPJ) were formulated into MW solution with different concentrations of peppermint oil. MW formulations were assessed for physical appearance, stability, and anti-microbial activities. Results: About 2% of peppermint oil in FPJ was found as organoleptically optimum. The pH and refractive indexes of the MWs were not affected during storage and stability assessments. All the FPJ-MWs formulations showed antimicrobial activity against Group A Staphylococcus, and other oral pathogens - Escherichia coli, Streptococcus aureus, and Pseudomonas aeruginosa. Moreover, black galingale, mangosteen, and noni based MW formulas also exhibited anti-candida activity. The MW made from fermented black galingale (Kaempferia parviflora) juice was the most potent antimicrobial formulation with excellent physical stability. Conclusion: The study concluded that fermented plant-herbal juices can be used as natural MW recipe with 2% of peppermint oil to improve the flavor and aroma. The formulations were stable, free of microbial contamination, and also exhibited antimicrobial activity. Further extended stability study and clinical trials are necessary to develop a commercial FPJ-based natural MW recipe.