Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorRômulo F. Duarteen_US
dc.contributor.authorChanakan Prom-u-thaien_US
dc.contributor.authorDouglas C. Amaralen_US
dc.contributor.authorValdemar Faquinen_US
dc.contributor.authorLuiz R.G. Guilhermeen_US
dc.contributor.authorAndré R. Reisen_US
dc.contributor.authorEduardo Alvesen_US
dc.description.abstract© 2015 Elsevier Ltd. Dithizone (DTZ) staining is a rapid, simple, and inexpensive method that allows the histochemical localization of Zn. In this study, we evaluated genotypic variation in Zn concentration in brown rice grown in the field using atomic absorption spectrophotometer (AAS) as DTZ staining quantified using ImageJ software. We used dehusked grains from upland rice accessions widely cultivated in Brazil. The DTZ staining showed that the concentration of Zn varied within and between rice grains. The staining intensity index (Y) provided differences in localization of Zn across the grain regions. Zn concentration in brown rice varied in multiple regression analysis, showing major differences in index weighted by stained area of each region (YAW) for the embryo, endosperm, and aleurone, especially in the endosperm and aleurone regions due to a large portion of the area in the kernel. The total YAW among rice accessions was positively correlated with Zn concentration in grains by chemical analysis. The DTZ staining associated with imageJ software is a promising to estimate Zn concentration in different grain tissues. Thus, this method may be useful for rapid screening of rice germplasms.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleDetermination of zinc in rice grains using DTZ staining and ImageJ softwareen_US
article.title.sourcetitleJournal of Cereal Scienceen_US
article.volume68en_US Mai Universityen_US Federal de Lavrasen_US Estadual Paulistaen_US
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.