An improved method for purification of marine cyanobacteria isolated from the Gulf of Thailand

Abstract

© 2015, Journal of Pure and Applied Microbiology. All rights reserved. Thirty five marine cyanobacteria isolates obtained from water samples collected from the Gulf of Thailand of the Chumpon and Chonburi provinces. They were purified by homogenizing, washing, lysozyme, antibiotics and dilution. Successful purifications were checked by morphological characterization (plate culture and scanning electron microscope) and molecular methods (Multiplex randomly amplified polymorphic DNA and 16S rRNA gene). This study presents the results of axenic cyanobacteria were obtained on ASNIII agar plates and scanning electron microscopy. The DNA fingerprints of all axenic marine cyanobacteria were divided into eight clusters and identified using 16S rRNA gene sequence correlatively close to Geitlerinema sp., Leptolyngbya valdenosa, Leptolyngbya sp., Synechococcus sp., Pseudanabaena limnetica (Oscillatoria limnetica) and Oscillatoriales cyanobacterium.