Jiangella mangrovi sp. nov., isolated from mangrove soil

Abstract

© 2015 IUMS. An aerobic, Gram-stain-positive actinomycete, designated strain 3SM4-07^T, was characterized using a polyphasic taxonomic approach. The strain produced branching mycelium which fragmented into short or elongated rods. The whole-cell hydrolysates contained ll-2,6-diaminopimelic acid as the diagnostic diamino acid, with glucose and ribose as the main sugars. The predominant cellular fatty acids were anteiso-C<inf>15: 0</inf>, iso-C<inf>15: 0</inf> and iso-<inf>C16: 0</inf>. The predominant menaquinone was MK-9(H<inf>4</inf>). Phospholipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and phosphatidylinositol mannoside. Mycolic acids were absent. The DNA G + C content was 72.3 mol%. Strain 3SM4-07^T formed a phylogenetic line within the genus Jiangella and its 16S rRNA gene sequence was related most closely to Jiangella alkaliphila D8-87T (99.0% similarity), Jiangella muralis 15-Je-01 7^T (98.8%), Jiangella alba YIM 61503^T (98.6%) and Jiangella gansuensis YIM 002^T (98.6%). However, mean DNA-DNA hybridization values revealed that strain 3SM4-07^T differed from the closest species previously described in this genus. Data from phenotypic, chemotaxonomic and molecular analyses between strain 3SM4-07^T andrecognizedspecies of the genus Jiangella indicate that strain 3SM4-07^T is a representative of a novel species of the genus Jiangella, for which the name Jiangella mangrovi sp. nov. is proposed. The type strain is 3SM4-07^T (=BCC 60398^T=NBRC 109648^T).