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dc.contributor.authorL. D. Yuen_US
dc.contributor.authorK. Sangwijiten_US
dc.contributor.authorK. Prakrajangen_US
dc.contributor.authorB. Phanchaisrien_US
dc.contributor.authorP. Thongkumkoonen_US
dc.contributor.authorP. Thopanen_US
dc.contributor.authorS. Singkaraten_US
dc.contributor.authorS. Anuntalabhochaien_US
dc.description.abstractAs a useful ion beam biotechnology, ion-bombardment-induced DNA transfer into bacterial Escherichia coli (E. coli) cells has been successfully operated using argon ions. In the process ion bombardment of the bacterial cells modifies the cell envelope materials to favor the exogenous DNA molecules to pass through the envelope to enter the cell. The occurrence of the DNA transfer induction was found ion energy and fluence dependent in a complex manner. At ion energy of a few keV and a few tens of keV to moderate fluences the DNA transfer could be induced by ion bombardment of the bacterial cells, while at the same ion energy but to high fluences DNA transfer could not be induced. On the other hand, when the ion energy was medium, about 10-20 keV, the DNA transfer could not be induced by ion bombardment of the cells. The complexity of the experimental results indicated a complex mechanism which should be related to the complex structure of the bacterial E. coli cell envelope. A phase diagram was proposed to interpret different mechanisms involved as functions of the ion energy and fluence. © 2014 Elsevier B.V. All rights reserved.en_US
dc.subjectPhysics and Astronomyen_US
dc.titleMechanisms of ion-bombardment-induced DNA transfer into bacterial E. coli cellsen_US
article.title.sourcetitleNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atomsen_US
article.volume326en_US on Higher Educationen_US Mai Universityen_US Universityen_US
Appears in Collections:CMUL: Journal Articles

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