Culture conditions and some properties of pure culture of ectomycorrhizal fungus, Scleroderma sinnamariense

Abstract

Scleroderma are ectomycorrhizal fungi which have a worldwide distribution. In 2010, basidiomes of Scleroderma sp. CMUS01 were collected from northern Thailand and pure cultures were obtained. Samples were identified based on morphology and molecular characteristics as Scleroderma sinnamariense. In this paper we report the optimum in vitro culture conditions of S. sinnamariense. Among ten culture media tested, fungal-host agar was the best medium for the mycelial growth and biomass yield. The fungus was able to grow at temperatures ranging from 20-35°C, with an optimal growth temperature of 30°C. The optimal pH for mycelial growth was 5. 0. In addition, this strain produced indole-3-acetic acid and siderophore in pure culture. This report provides valuable information for S. sinnamariense mycelial cultivation in Thailand.