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dc.contributor.authorSiripan Songsawatkiaten_US
dc.contributor.authorVisut Baimaien_US
dc.contributor.authorSorawat Thongsahuanen_US
dc.contributor.authorYasushi Otsukaen_US
dc.contributor.authorKritsana Taaien_US
dc.contributor.authorChayanit Hempolchomen_US
dc.contributor.authorWichai Srisukaen_US
dc.contributor.authorPetchaboon Poolpholen_US
dc.contributor.authorWej Choochoteen_US
dc.contributor.authorAtiporn Saeungen_US
dc.contributor.authorIgor Sharkhoven_US
dc.description.abstract© The Author 2014. Metaphase karyotype investigation on two allopatric strains of Anopheles nitidus Harrison, Scanlon, and Reid (Diptera: Culicidae) was conducted in Thailand during 2011-2012. Five karyotypic forms, i.e., Form A (X1, Y1), Form B (X1, Y2), Form C (X2, Y3), Form D (X1, X3, Y4), and Form E (X1, X2, X3, Y5) were obtained from a total of 21 isofemale lines. Forms A, B, and C were confined to Phang Nga Province, southern Thailand, whereas Forms D and E were restricted to Ubon Ratchathani Province, northeastern Thailand. Cross-mating experiments among the five isofemale lines, which were representative of five karyotypic forms of An. nitidus, revealed genetic compatibility by providing viable progenies and synaptic salivary gland polytene chromosomes through F2generations. The results suggest that the forms are conspecific, and An. nitidus comprises five cytological races. The very low intraspecific sequence variations (average genetic distances = 0.002-0.008) of the nucleotide sequences in ribosomal DNA (internal transcribed spacer 2) and mitochondrial DNA (cytochrome c oxidase subunits I and II) among the five karyotypic forms were very good supportive evidence.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.titleEvidence to support a conspecific nature of allopatric cytological races of Anopheles nitidus (Diptera: Culicidae) in Thailanden_US
article.title.sourcetitleJournal of Insect Scienceen_US
article.volume14en_US Mai Universityen_US Universityen_US of Songkla Universityen_US Universityen_US Sectionen_US Ministry of Public Healthen_US
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