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dc.contributor.authorTongjit Thanchomnangen_US
dc.contributor.authorPewpan M. Intapanen_US
dc.contributor.authorViraphong Lulitanonden_US
dc.contributor.authorSomboon Sangmaneedeten_US
dc.contributor.authorSudchit Chungpivaten_US
dc.contributor.authorPiyanan Taweethavonsawaten_US
dc.contributor.authorWej Choochoteen_US
dc.contributor.authorWanchai Maleewongen_US
dc.description.abstractA real-time fluorescence resonance energy transfer (FRET) PCR supplemented with melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito vectors and dog blood samples was developed. This real-time FRET PCR was based on the fluorescence melting curve analysis of a hybrid between an amplicon generated from the D. immitis ribosomal RNA gene sequence and specific fluorophore-labeled probes. The sensitivity, specificity, accuracy, and positive and negative predictive values of this method were all 100%. Besides being highly sensitive and specific, this PCR is fast and offers a high throughput. Therefore it is a suitable and powerful tool for the diagnosis and for epidemiological surveys of canine dirofilariasis as well as for molecular xenomonitoring of D. immitis in mosquito vectors. © 2009 Elsevier B.V. All rights reserved.en_US
dc.subjectImmunology and Microbiologyen_US
dc.titleRapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysisen_US
article.title.sourcetitleVeterinary Parasitologyen_US
article.volume168en_US Kaen Universityen_US of Medicine, Khon Kaen Universityen_US Universityen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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