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dc.contributor.authorRapiphun Janmaneeen_US
dc.contributor.authorAkira Babaen_US
dc.contributor.authorSukon Phanichphanten_US
dc.contributor.authorSaengrawee Sriwichaien_US
dc.contributor.authorKazunari Shinboen_US
dc.contributor.authorKeizo Katoen_US
dc.contributor.authorFutao Kanekoen_US
dc.description.abstractAn electrochemically controlled surface plasmon resonance (SPR) immunosensor for the detection of human immunoglobulin G (IgG) has been developed using poly(pyrrole-3-carboxylic acid) (PP3C) film. In this work, a pyrrole-3-carboxylic acid monomer was used for electropolymerization of a PP3C film on a gold-coated high-refractive-index glass slide. In situ electrochemical (EC)-SPR spectroscopy was performed to study the kinetic property and electroactivity property of the PP3C film. Moreover, ultraviolet-visible (UV-vis) spectroscopy was performed to characterize the PP3C film. Finally, the immunosensor-based PP3C film was constructed. The carboxylic acid surface of the PP3C film was activated for the immobilization of anti-human IgG. The immunosensor electrode was used for probing the binding reaction of anti-human IgG/human IgG with several concentrations of human IgG at different constant applied potentials. The probe immobilization and immunosensing process were in situ monitored by EC-SPR technique. The sensitivity of the sensor was improved by controlling the morphology of the PP3C film by applying the potential. © 2011 The Japan Society of Applied Physics.en_US
dc.subjectPhysics and Astronomyen_US
dc.titleDetection of human IgG on Poly(pyrrole-3-carboxylic acid) thin film by electrochemical-Surface plasmon resonance spectroscopyen_US
article.title.sourcetitleJapanese Journal of Applied Physicsen_US
article.volume50en_US Universityen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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