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dc.contributor.authorVoravuth Somsaken_US
dc.contributor.authorSomdet Srichairatanakoolen_US
dc.contributor.authorSumalee Kamchonwongpaisanen_US
dc.contributor.authorYongyuth Yuthavongen_US
dc.contributor.authorChairat Uthaipibullen_US
dc.description.abstractThe standard protocol for genetic modification of the rodent malaria parasite Plasmodium berghei requires infected blood from one or more laboratory mice, followed by large-scale in vitro parasite culture and purification of mature schizonts. Here, protocols are described for small-scale in vitro culture from 20 μL of mouse tail blood and purification of mature P. berghei schizonts sufficient for a single transfection experiment. All procedures are performed in 1.5-mL microcentrifuge tubes. We confirmed that transgenic parasites could be obtained using schizonts prepared by this protocol. This small-scale protocol provides significant advantages, namely reduction of parasite sample, laboratory consumables and mice for transfection experiments. © 2011 Elsevier B.V. All rights reserved.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleSmall-scale in vitro culture and purification of Plasmodium berghei for transfection experimenten_US
article.title.sourcetitleMolecular and Biochemical Parasitologyen_US
article.volume177en_US National Center for Genetic Engineering and Biotechnologyen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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