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dc.contributor.authorPornsiri Pitchakarnen_US
dc.contributor.authorShugo Suzukien_US
dc.contributor.authorKumiko Ogawaen_US
dc.contributor.authorWilart Pompimonen_US
dc.contributor.authorSatoru Takahashien_US
dc.contributor.authorMakoto Asamotoen_US
dc.contributor.authorPornngarm Limtrakulen_US
dc.contributor.authorTomoyuki Shiraien_US
dc.description.abstractIn this study, we focused on the effects of a bitter melon (Momordica charantia) leaf extract (BMLE) and a purified component, Kuguacin J (KuJ), on androgen-dependent LNCaP human prostate cancer cells. Both treatments exerted growth inhibition through G1 arrest and induction of apoptosis. In addition, KuJ markedly decreased the levels of cyclins (D1 and E), cyclin-dependent kinases (Cdk2 and Cdk4) and proliferating cell nuclear antigen, and caused an increase in p21 and p27 levels. Its induction of apoptosis was accompanied by an increase in cleavage of caspase-3 and poly (ADP-ribose) polymerase, attributable to augment of Bax/Bcl-2 and Bad/Bcl-xL and reduction of survivin levels. BMLE and KuJ also reduced the expression of androgen receptor (AR), prostate-specific antigen (PSA) while induced P53 protein level. Down-regulation of p53 by RNA interference indicated that BMLE and KuJ inhibited cell growth partly through p53-dependent cell cycle arrest and apoptotic pathways. Both BMLE and KuJ caused less toxicity in a normal prostate cell line, PNT1A. Our results suggest that BMLE and a purified component, KuJ, from its diethyl ether fraction could be promising candidate new antineoplastic and chemopreventive agents for androgen-dependent prostate cancer and carcinogenesis. © 2011 Elsevier Ireland Ltd.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleInduction of G1 arrest and apoptosis in androgen-dependent human prostate cancer by Kuguacin J, a triterpenoid from Momordica charantia leafen_US
article.title.sourcetitleCancer Lettersen_US
article.volume306en_US City Universityen_US Institute of Health Sciences Tokyoen_US Universityen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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