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dc.contributor.authorPiyachai Premvaranonen_US
dc.contributor.authorSuchada Vearasilpen_US
dc.contributor.authorSa nguansak Thanapornpoonpongen_US
dc.contributor.authorDumnern Karladeeen_US
dc.contributor.authorShela Gorinsteinen_US
dc.description.abstractThe aim of this investigation was to improve in vitro the technique of production of double haploid in Indica hybrid rice by combining anther culture, hormone shock and doubling chromosome. It was discussed how to avoid somaclonal variation during culturing and to reduce the time of this process. The anthers of KDML 105 × SPR 1 (Indica × Indica) were cultured in Linsmaier and Skoog (LS) medium, which contained nutrients, growth regulators [(2,4,-dichlorophenoxy acetic acid (2,4-D) and naphthalene acetic acid (NAA)] and organic compounds, and then subcultured by inducing embryo-like structure (ELS) LS media. During 4 weeks used LS media supplemented with 10 μM KNO3+ 2 mg/L 2,4-D + 2 mg/L NAA + 20% coconut water + 1 mg/L of activated charcoal had induced high embryogenic frequent callus with length of 4-5 mm. The supplementation of 0.2 g/L colchicine and 100 μM 2,4-D was the most efficient in LS media. Over 70% of viable double haploid ELS were produced in 8 weeks and subcultured only twice compared with conventional anther which takes more than 12 weeks. This new technique can therefore be applied to rice in order in shorten time to produce higher number of double haploid plantlets. © 2011 Versita Warsaw and Springer-Verlag Wien.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleIn vitro studies to produce double haploid in Indica hybrid riceen_US
article.volume66en_US Mai Universityen_US of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE)en_US on Higher Educationen_US University-Hadassah Medical Schoolen_US
Appears in Collections:CMUL: Journal Articles

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