Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/46009
Title: The Role of Laccase Enzymes of Penicillium marneffei on Cytokine Production in Macrophage
Other Titles: บทบาทของเอนไซม์แลคเคสของเชื้อ Penicillium marneffei ต่อการสร้างไซโตไคน์ของมาโครฟาจ
Authors: สิริดา ยังฉิม
นงนุช วณิตย์ธนาคม
พจนา ศรีบุรี
จุติกุล แก้วมาลากุล
Jutikul Kaewmalakul
Keywords: Laccase enzymes
Penicillium marneffei
Cytokine production
Issue Date: Sep-2014
Publisher: เชียงใหม่ : บัณฑิตวิทยาลัย มหาวิทยาลัยเชียงใหม่
Abstract: Penicillium marneffei has emerged as an opportunistic dimorphic fungus associated with Human Immunodeficiency Virus (HIV), particularly in the geographical area of endemicity in Southeast Asia and southern China. Upon host infection, one of the most important aspects that contributes to the disease outcome is the initial interaction of the P. marneffei conidia with the phagocytic cells and the induction of the inflammatory process. Laccase enzyme has been found to associate with virulence in several pathogenic fungi. To obtain a better understanding of a role of laccase in P. marneffei, conidia of laccase mutant strains or wild-type were co-cultured with THP-1, human monocyte leukemia cell line in different incubation time. The percentage of phagocytosis and phagocytic index of quadruple lac gene deletion strain were significantly higher than the wild-type at 1 and 2 hours of incubation. In addition, the significantly higher percentage of intracellular killing of the quadruple lac gene deletion strain was observed when compared with wild-type at 4 and 8 hours of incubation. The pro-inflammatory cytokines, TNF-α, IL-1β and IL-6, secreted from THP-1 infected with the quadruple lac gene deletion strain were significantly increased in comparing with the wild-type. Altogether, these results defined the role of laccase that promoted P. marneffei resistance to innate host defenses.
URI: http://cmuir.cmu.ac.th/jspui/handle/6653943832/46009
Appears in Collections:MED: Theses

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APPENDIX.pdfAPPENDIX568.55 kBAdobe PDFView/Open    Request a copy
CHAPTER 1.pdfCHAPTER 1181.86 kBAdobe PDFView/Open    Request a copy
CHAPTER 2.pdfCHAPTER 2444.97 kBAdobe PDFView/Open    Request a copy
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CHAPTER 6.pdfCHAPTER 6212.58 kBAdobe PDFView/Open    Request a copy
CHAPTER 7.pdfCHAPTER 7197.8 kBAdobe PDFView/Open    Request a copy
CONTENT.pdfCONTENT177.52 kBAdobe PDFView/Open    Request a copy
COVER.pdfCOVER532.35 kBAdobe PDFView/Open    Request a copy
REFERENCE.pdfREFERENCE510.21 kBAdobe PDFView/Open    Request a copy


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