ความหลากหลายของสายพันธุ์ต้นพะยูงในเขตภาคตะวันออกและภาคตะวันออกเฉียงเหนือในไทยด้วยเครื่องหมายดีเอ็นเอบริเวณ trnL-trnL และ trnL-trnF

Abstract

Siamese rosewood is now a list of endangered species according to illegal logging. Rosewood is a hardwood with a beautiful pattern. For this reason, it is used to make expensive furniture and wood carving. This research aims to study the diversity of Siamese rosewood (Dalbergia cochinchinensis Pierre.) in Eastern and Northeastern of Thailand using trnL-trnLand trnL-trnF regionsin chloroplast DNA as genetic markers. A total of thirty nine young leave samples from seven provinces in Thailand, including Ubon Ratchathani, Udon Thani, Khon Kaen, Si Sa Ket, Sakonnakhon, Phitsanulok and Chachoengsao were used. Nucleotide sequences in both intergenic spacer regions showed that one sample from Ubon Ratchathani and Chachoengsao (UB05 and CC04) presented a tranversion (A→C) in the trnL-trnL region at position 138. All nucleotide sequences of the samples were compared with the NCBI database using BLAST program. To analyze the evolutionary classification, Molecular evolutionary genetics analysis (MEGA) program with Neighbor-Joining Tree (NJtree) method were performed. It was found that nucleotide sequences of the samples were similar to chloroplast DNA from Dalbergia cochinchinensis voucher VNMN:B0001132 and Dalbergia lanceolaria voucher Sirichamorn YSM 2009-02 with maximum identities score of100% and 96% in trnL-trnL and trnL-trnF regions, respectively. However, Siamese rosewood samples which were collected from seven provinces could not be classified when using trnL-trnL and trnL-trnF regions as genetic markers. Furthermore, the different genetic markers should be applied to analyze the genetic diversity of Siamese rosewood within and different geographical areas.