ผลของการเสริมสารสกัดจากไม้ฝางต่อประสิทธิภาพการผลิตของไก่ไข่และการแสดงออกของยีน

Abstract

The present study investigated the effects of Caesalpinia sappan extract (CSE) supplementation on productive performance and gene expression of laying hens. Three hundred and sixty (64-week-old) Hy-line Brown hens were divided into five groups with 0 (control diet), 250, 500, 1000, and 2000 mg/kg CSE, respectively. Subsequently, the analyses were conducted on productive performance, egg quality, small intestinal histology, and antioxidant and inflammatory cytokines-related gene expression. The results shown that supplementation with CS2000 led to increase in egg weight (P<0.05). CS500, CS1000, and CS2000 could let to increase egg mass (P<0.05). Supplementation with CSE extract led to decrease the feed conversion ratio in 5-8 weeks of the experiment (P<0.05) and tended to decrease the feed conversion ratio in 1-8 weeks of the experiment. Moreover, CS1000 and CS2000 led to increase in albumin weight (P<0.05). The morphology of the small intestinal tissue in CS2000 had the highest value (P<0.05), and tended to suggesting an increase in the villus height to crypt depth ratio. It was also found that the expression of genes that stimulate inflammation were down regulated. Meanwhile, expression of genes related to antioxidant and anti-inflammatory actions were up regulated in the liver, ileum, and magnum (P<0.05). Therefore, it can be seen that when testing the efficiency of CSE extract at the level of 2,000 mg/kg food, it can improve production efficiency, egg quality, and intestinal morphology. Including the expression of genes related to antioxidants and cytokines that stimulate inflammation in the diets of laying hens in the late phase of the laying cycle. Consequently, it can be considered that the bioactive components of Sappan wood extract aid in the defense against free radicals, the reduction of inflammation, and the overall improvement of the small intestine of the hens. Incorporating CSE into basal diets for laying hens at late laying stage contributes to an overall improvement in egg quality and productive performance.