การชักนำการออกดอกของกล้วยไม้เอื้องนางลมในหลอดทดลอง

Abstract

Dendrobium peguanum Lindl. is a small orchid that distributes in India, Nepal, Myanmar, and Thailand. This research aimed to use tissue culture technique with some plant growth regulators for in vitro propagation and flowering. After pollination, seed pods of Den. peguanum Lindl. Were harvested at 6, 8, 10 and 12 weeks. Seed viability was evaluated by lacto-propionic orcein staining, and then seeds were cultured on Murashige and Skoog medium (MS) without plant growth regulator. It was found that seeds from 6-week-old seed pods could not germinate while those at 10-week-old could germinate well. After that, seedlings were subcultured on ½ MS medium containing some PRGs i.e. α-naphthalene acetic acid (NAA), 6-benzylaminopurine (BA), kinetin and coconut water. It was found that plants grown on media with NAA 0.5 and 1.0 ppm at 120 days gave better growth in terms of height than those of other treatments whereas number of shoots per plant and number of leaves were not significantly different. Plants were subcultured on a new ½ MS medium containing some PGRs for in vitro flowering induction. It was found that plants on media with BA 1.0, 1.5 and 2.0 ppm could flower but plants on other PGRs had no flower.