Red jasmine rice extract suppresses genes associated with cartilage degradation in IL-1β-stimulated chondrosarcoma (SW1353) via blocking NF-κB pathway

Abstract

Objective This study aimed to evaluate the chondroprotective potential of red jasmine rice crude extract (RRE) against an expression of genes associated with cartilage degradation stimulated by IL-1β in a human chondrosarcoma cell line, SW1353, culture model. Methods SW1353 cells were pre-incubated with 1 ng/mL of recombinant human interleukin-1β (IL-1β) followed by the addition of RRE in a range of concentrations from of 0 to 100 μg/mL. After 24 hours of incubation, cells were harvested and expression of genes associated with cartilage degradation was measured using real-time RT-PCR. The culture media were evaluated for MMP-13 levels using an ELISA kit. Upregulation of the signaling pathway which promotes the infl ammatory cascade, nuclear factor kappa B (NF-κB), was determined by Western blot analysis. Results Cytokine IL-1β signifi cantly upregulated the expression of the proinfl ammatory mediators IL-1β, IL-6, TNF-α, and COX-2 as well as enzymes involved in osteoarthritis such as MMP-13, ADAMTS-4, and ZIP-8. The results showed that RRE at a concentration of 100 μg/mL signifi cantly suppressed IL-1β-induced expression of these genes. Moreover, the effect of IL-1β on NF-κB signaling pathway activation was suppressed by RRE via decreased phosphorylated form of IKKα/β, IκBα, and NF-κB p65. Conclusion RRE has chondroprotective potential through reduction of the gene upregulating effect of IL-1β which involve cartilage degradation via suppression of the NF-κB signaling pathway. Development of RRE has for alternative use potential in treating degenerative joint diseases.