Please use this identifier to cite or link to this item:
Title: Identification of human hnRNP C1/C2 as a dengue virus NS1-interacting protein
Authors: Sansanee Noisakran
Suchada Sengsai
Visith Thongboonkerd
Rattiyaporn Kanlaya
Supachok Sinchaikul
Shui Tein Chen
Chunya Puttikhunt
Watchara Kasinrerk
Thawornchai Limjindaporn
Wiyada Wongwiwat
Prida Malasit
Pa thai Yenchitsomanus
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 18-Jul-2008
Abstract: Dengue virus nonstructural protein 1 (NS1) is a key glycoprotein involved in the production of infectious virus and the pathogenesis of dengue diseases. Very little is known how NS1 interacts with host cellular proteins and functions in dengue virus-infected cells. This study aimed at identifying NS1-interacting host cellular proteins in dengue virus-infected cells by employing co-immunoprecipitation, two-dimensional gel electrophoresis, and mass spectrometry. Using lysates of dengue virus-infected human embryonic kidney cells (HEK 293T), immunoprecipitation with an anti-NS1 monoclonal antibody revealed eight isoforms of dengue virus NS1 and a 40-kDa protein, which was subsequently identified by quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) as human heterogeneous nuclear ribonucleoprotein (hnRNP) C1/C2. Further investigation by co-immunoprecipitation and co-localization confirmed the association of hnRNP C1/C2 and dengue virus NS1 proteins in dengue virus-infected cells. Their interaction may have implications in virus replication and/or cellular responses favorable to survival of the virus in host cells. © 2008 Elsevier Inc. All rights reserved.
ISSN: 10902104
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.