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dc.contributor.authorSansanee Noisakranen_US
dc.contributor.authorSuchada Sengsaien_US
dc.contributor.authorVisith Thongboonkerden_US
dc.contributor.authorRattiyaporn Kanlayaen_US
dc.contributor.authorSupachok Sinchaikulen_US
dc.contributor.authorShui Tein Chenen_US
dc.contributor.authorChunya Puttikhunten_US
dc.contributor.authorWatchara Kasinrerken_US
dc.contributor.authorThawornchai Limjindapornen_US
dc.contributor.authorWiyada Wongwiwaten_US
dc.contributor.authorPrida Malasiten_US
dc.contributor.authorPa thai Yenchitsomanusen_US
dc.description.abstractDengue virus nonstructural protein 1 (NS1) is a key glycoprotein involved in the production of infectious virus and the pathogenesis of dengue diseases. Very little is known how NS1 interacts with host cellular proteins and functions in dengue virus-infected cells. This study aimed at identifying NS1-interacting host cellular proteins in dengue virus-infected cells by employing co-immunoprecipitation, two-dimensional gel electrophoresis, and mass spectrometry. Using lysates of dengue virus-infected human embryonic kidney cells (HEK 293T), immunoprecipitation with an anti-NS1 monoclonal antibody revealed eight isoforms of dengue virus NS1 and a 40-kDa protein, which was subsequently identified by quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) as human heterogeneous nuclear ribonucleoprotein (hnRNP) C1/C2. Further investigation by co-immunoprecipitation and co-localization confirmed the association of hnRNP C1/C2 and dengue virus NS1 proteins in dengue virus-infected cells. Their interaction may have implications in virus replication and/or cellular responses favorable to survival of the virus in host cells. © 2008 Elsevier Inc. All rights reserved.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleIdentification of human hnRNP C1/C2 as a dengue virus NS1-interacting proteinen_US
article.title.sourcetitleBiochemical and Biophysical Research Communicationsen_US
article.volume372en_US National Center for Genetic Engineering and Biotechnologyen_US of Medicine, Siriraj Hospital, Mahidol Universityen_US Research Center, Academia Sinicaen_US Taiwan Universityen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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