Please use this identifier to cite or link to this item:
http://cmuir.cmu.ac.th/jspui/handle/6653943832/55894
Title: | Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus |
Authors: | Ingrid A. Beck Rachel Payant Nicole Ngo-Giang-Huong Woottichai Khamduang Laddawan Laomanit Gonzague Jourdain Lisa M. Frenkel |
Authors: | Ingrid A. Beck Rachel Payant Nicole Ngo-Giang-Huong Woottichai Khamduang Laddawan Laomanit Gonzague Jourdain Lisa M. Frenkel |
Keywords: | Immunology and Microbiology |
Issue Date: | 1-Jul-2016 |
Abstract: | © 2016 Elsevier B.V. Treatment of chronic hepatitis B virus (HBV) infection with lamivudine-monotherapy rapidly selects mutant variants in a high proportion of individuals. Monitoring lamivudine resistance by consensus sequencing is costly and insensitive for detection of minority variants. An oligonucleotide ligation assay (. OLA) for HBV lamivudine-resistance was developed and compared to consensus sequencing. Both assays detected drug resistance mutations in 35/64 (54.7%) specimens evaluated, and OLA detected minority mutants in an additional six (9.4%). OLA may offer a sensitive and inexpensive alternative to consensus sequencing for detection of HBV drug resistance in resource-limited settings. |
URI: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84962283790&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/55894 |
ISSN: | 18790984 01660934 |
Appears in Collections: | CMUL: Journal Articles |
Files in This Item:
There are no files associated with this item.
Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.