Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/55149
Title: Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis
Authors: Witida Laopajon
Nuchjira Takheaw
Watchara Kasinrerk
Supansa Pata
Keywords: Biochemistry, Genetics and Molecular Biology
Health Professions
Immunology and Microbiology
Medicine
Issue Date: 2-Sep-2016
Abstract: © 2016 Taylor & Francis. The current available assays cannot differentiate the stages of phagocytosis. We, therefore, established methods for concurrent detection of antigen attachment and engulfment by phagocyte using latex beads coated with lipopolysaccharide, rabbit IgG, and carboxyfluorescein diacetate succinimidyl ester. The generated beads were incubated with whole blood at 37°C for 1 hr and stained with PE-Cy5.5 anti-rabbit IgG antibody. By flow cytometry, attachment and phagocytic processes could be detected, simultaneously. The established method is a valuable tool for diagnosis of phagocytic disorder and study of molecules involved in phagocytosis.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84975512237&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/55149
ISSN: 15324230
15321819
Appears in Collections:CMUL: Journal Articles

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