Please use this identifier to cite or link to this item:
Title: Response surface optimization of exopolysaccharide production from sugarcane juice by Lactobacillus confusus TISTR 1498
Authors: Phayungsak Manochai
Yuthana Phimolsiripol
Phisit Seesuriyachan
Keywords: Multidisciplinary
Issue Date: 1-Jan-2014
Abstract: Lactobacillus confusus TISTR 1498, isolated from Thai traditional fermented pork (Nham), could secrete large amounts of exopolysaccharides (EPS). Response surface methodology was applied to optimize the culture conditions for EPS production using Central Composite Design (CCD). The effects of three variables including pH (4-7), temperature (20-40°C) and nitrogen source (0.25-1.75 PYB) on EPS yield and biomass were investigated. The 1PYB was the mixtures of 5 g/L peptone (P), 2.5 g/L yeast extract (Y) and 2.5 g/L beef extract (B). Response surface methodology showed that the data were adequately fitted to a second-order polynomial model via quadratic regression relationships. The optimal culture conditions for EPS production in sugarcane juice were pH of 5.55, 29.75°C and 1.22PYB, which was composed of 6.1 g/L peptone, 3.05 g/L yeast extract and 3.05 g/L beef extract. Under the optimum condition, the predicted maximum EPS production was 107.5 g/L and the predicted biomass was 1.92 g/L. In submerged fermentation, sugarcane juice enhanced EPS yield twice as well as the modified MRS sucrose medium. In addition, the cost of medium can be lowered to 0.53 THB/g EPS, which was lower than that of the medium from the mixtures of the PYB (0.78 THB/gEPS).
ISSN: 16851994
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.