Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/51354
Title: Clonality Despite Sex: The Evolution of Host-Associated Sexual Neighborhoods in the Pathogenic Fungus Penicillium marneffei
Authors: Daniel A. Henk
Revital Shahar-Golan
Khuraijam Ranjana Devi
Kylie J. Boyce
Nengyong Zhan
Natalie D. Fedorova
William C. Nierman
Po Ren Hsueh
Kwok Yung Yuen
Tran P.M. Sieu
Nguyen Van Kinh
Heiman Wertheim
Stephen G. Baker
Jeremy N. Day
Nongnuch Vanittanakom
Elaine M. Bignell
Alex Andrianopoulos
Matthew C. Fisher
Keywords: Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Issue Date: 1-Oct-2012
Abstract: Molecular genetic approaches typically detect recombination in microbes regardless of assumed asexuality. However, genetic data have shown the AIDS-associated pathogen Penicillium marneffei to have extensive spatial genetic structure at local and regional scales, and although there has been some genetic evidence that a sexual cycle is possible, this haploid fungus is thought to be genetically, as well as morphologically, asexual in nature because of its highly clonal population structure. Here we use comparative genomics, experimental mixed-genotype infections, and population genetic data to elucidate the role of recombination in natural populations of P. marneffei. Genome wide comparisons reveal that all the genes required for meiosis are present in P. marneffei, mating type genes are arranged in a similar manner to that found in other heterothallic fungi, and there is evidence of a putatively meiosis-specific mutational process. Experiments suggest that recombination between isolates of compatible mating types may occur during mammal infection. Population genetic data from 34 isolates from bamboo rats in India, Thailand and Vietnam, and 273 isolates from humans in China, India, Thailand, and Vietnam show that recombination is most likely to occur across spatially and genetically limited distances in natural populations resulting in highly clonal population structure yet sexually reproducing populations. Predicted distributions of three different spatial genetic clusters within P. marneffei overlap with three different bamboo rat host distributions suggesting that recombination within hosts may act to maintain population barriers within P. marneffei. © 2012 Henk et al.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84868103431&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51354
ISSN: 15537374
15537366
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.


Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.