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Title: Fed-batch Medium Modification for Secreted Single Chain Variable Fragment Against HIV-1 p17 Protein Production Using Combined Two Factorial Experiments in Escherichia coli Bioreactor
Other Titles: การปรับปรุงอาหารเลี้ยงเชื้อแบบกึ่งกะเพื่อผลิตซิงเกิลเชน แวริเอเบิลแฟรกเมนต์ แบบคัดหลั่งต่อโปรตีน p17 ของเชื้อเอชไอวี-1 จากเชื้อเอชเชอริเชีย โคไล ในถังชีวปฏิกรณ์โดยใช้การทดลองแบบแฟคทอเรียลสองวิธีร่วมกัน
Authors: Asst. Prof. Dr. Bordin Butr-Indr
Prof.Dr. Chatchai Tayapiwatana
Asst. Prof. Dr. Phisit Seesuriyachan
Porntip Paopang
Keywords: HIV (Viruses)
Issue Date: 10-Apr-2014
Publisher: เชียงใหม่ : บัณฑิตวิทยาลัย มหาวิทยาลัยเชียงใหม่
Abstract: Biopharmaceutical production programs have been established using fed-batch cell culture protocols, which can support high volumetric productivity while providing low operational complexity. The industry is concerned with developing high-titer cell culture processes to meet increasing market demands and reduce manufacturing costs. Although advancements in bacterial cell engineering have enabled the development of high-performing recombinant cell lines, developments in cell culture media and process parameter settings are required to realize the maximum production of those cells. We investigated two combined optimization methods, Packlett-Burman design and Sequential Simplex Optimization, for optimization of feed medium. The new combined optimization method decreased the time required by 40.7% compared to the experiment trial. Dry cell weight was 1.24 times higher than in the individual method. The fed-batch cultivation with an optimal feeding rate of 20 ml/h increased cell growth, total protein production and scFv anti-p17 activity by 4.43, 1.48 and 6.5 times compared to batch cultivation respectively.
Appears in Collections:AMS: Theses

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