ผลของน้ำมันหอมระเหยเสม็ดขาวและตะไคร้ที่ผสมในตำรับน้ำยาบ้วนปากปราศจากแอลกอฮอล์ต่อการต้านแผ่นชีวภาพของราแคนดิดา อัลบิแคนส์บนพื้นผิวอะคริลิก

Abstract

The present study aimed to evaluate the efficacy of alcohol-free mouthwash containing cajuput (Melaleuca cajuputi Powell) and lemongrass (Cymbopogon citratus) essential oils and their synergistic action for eliminating biofilm-formed Candida albicans, the most common causative agent of denture stomatitis. The inhibitory activity against biofilm-formed C. albicans ATCC 10231 on 96-well plate and acrylic surfaces of this formula were significantly different from 0.12% chlorhexidine solution at 20 minutes (p ≤ 0.0001), and at 20 minutes and one hour of immersion (p ≤ 0.0001), respectively. At eight hours of immersion, the activity of this formula was similar to the activity of 0.12% chlorhexidine. Live cells and biofilm after treatment with this formula tended to be less densely active cells and biofilm than its negative control treatment and nearer to the activity of 0.12% chlorhexidine. The minimal inhibitory concentrations of cajuput and lemongrass essential oils were 2 and 4 µL/mL (0.2% and 0.4% v/v). For checkerboard assays, the fractional inhibitory concentration of these oils in the combination were 0.5 and 0.25 µL/mL, respectively. The combination of cajuput and lemongrass oils in this formula exhibited partial synergism against C. albicans ATCC 10231 with a fractional inhibitory concentration index of 0.75. This study demonstrated the inhibitory activity of this formula against biofilm-formed C. albicans on 96-well plate and acrylic surfaces after quantitated by colony enumeration and the XTT reduction assay resembled 0.12% chlorhexidine. It may be concluded that biofilm-formed C. albicans could be inhibited by the partial synergism of these essential oils in this mouthwash formula.