Development of multi-jet atmospheric pressure cold plasma for anti-aging

Abstract

The plasma jet technique, a popular method for generating reactive oxygen and nitrogen species through an inert gas such as argon mixing with air, was investigated. A plasma jet model was developed from a selection of statistical data, and the resulting NO, OH, and H2O2 concentrations were examined. The objective of the research was to establish a simple 2D plasma jet module and to mathematically understand the involved chemical reactions through the novelty of Multiphysics (finite element method). Determination of optimized conditions via design of experiment method was then performed. To create new responses, three nominal concentrations (NO, OH, and H2O2) were used to measure the performance of the species product. A full factorial design based on two levels, or 23 runs, with 3 center points and 2 replications, was employed in simulations using COMSOL Multiphysics 1) 5.3a software. The main effects and interactions between variables were identified for three main parameters: gas velocities of 40±70 m sí1 , plasma gaps of 10±30 mm, and simulation times of 2.8±4 ms. These parameters were selected to uncover design possibilities with external variables and can significantly contribute to the process of designing the prototype of a cell-scaled plasma jet model.

After simulation studied, the interested plasma jet discharge area is 10-20 mm from Fibroblast cells surface. DOE method was used for creating RONS concentrate predictions. This research focused on Air plasma which used to generate NO, O3 and H2O2. A full factorial design based on two levels, or 23 runs, with 3 center points and 3 replications, the responses of experiment are to optimize NO, O3 concentrations from plasma generated and to maximized %viability by MTT assay. The results shown that at plasma condition power level 3, air flow level 3 and 120 seconds can induce the highest % viability as 100.99 % at 72 hours incubate time with 9.46 % of error. the NO and O3 concentration models provided 7.8 ppm and 9.34 ppb, respectively. The error of the experimental results compared with the optimal value was 13.07 % and 0.68 %, respectively. This condition also uses for study telomerase enzyme induced. However, to confirm safety of the maximum condition from DOE method. Straining morphology from optical microscope and fluorescence with Apoptosis tested by flow cytometry are studied with cellular ROS in cell were tested at the same condition. The maximum condition did not damage to cells and can induce proliferation rate with unchanged in morphological. The telomerase enzyme level was investigated as significant cell aging parameter. The number of cells positive for telomerase enzyme was measured by RT-PCR. However, the results showed the interested trend in treated as 24, 48 and 72 hours incubated. The plasma treated seems to induce the telomerase level after 24 hours same as at 48 and 72 hours. There were induced in optimal plasma level 3 condition but not significant with the maximum telomerase dosed for 3.03 ng/ml after 48 hours and tends to be better after 72 hours incubated. The result showed the possibility of plasma to induced telomerase level and should be the new choice for anti-aging.