Evaluation of fiber efficiency in solid-phase microextraction for volatile organic compound extraction and identification in Caesalpinia mimosoides, Eleutherococcus trifoliatus and Persicaria odorata by coupling with gas chromatography-mass spectrometry

Abstract

Headspace solid-phase microextraction (HS-SPME) coupling with gas chromatography–mass spectrometry (GC–MS) was occupied for analysis of volatile organic compounds (VOCs) in northern Thai plants; Phak Pu Ya (Caesalpinia mimosoides), Phak Paem (Eleutherococcus trifoliatus) and Phak Phai (Persicaria odorata). HS-SPME fibers, including polydimethylsiloxane (PDMS), Carboxen®/ polydimethylsiloxane (CAR/PDMS), divinylbenzene/ Carboxen®/ polydimethylsiloxane (DVB/CAR/PDMS) and polyacrylate (PA), were used to evaluate the efficiency. It was found that each type flavored different chemical groups, increasing in phase of fiber seemed increasing its range of usage. DVB/CAR/PDMS had the widest range and was selected. Response surface methodology (RSM) models for each plant were established to determine the optimum conditions of VOCs extraction. RSM models had both precision and reliability. The main factors showed significance both individual and interaction. The combination of techniques obtained the optimum point. Full VOCs profiles of Caesalpinia mimosoides, Eleutherococcus trifoliatus and Persicaria odorata, with accurized 61 compounds, 142 compounds and 81 compounds, respectively, were observed. Major groups of volatile components were aldehydes and terpenoids, such as monoterpenes and sesquiterpenes.