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dc.contributor.authorJulaluk Khemacheewakulen_US
dc.contributor.authorSiraphat Taesuwanen_US
dc.contributor.authorRojarej Nuntaen_US
dc.contributor.authorCharin Techapunen_US
dc.contributor.authorYuthana Phimolsiripolen_US
dc.contributor.authorPornchai Rachtanapunen_US
dc.contributor.authorKittisak Jantanasakulwongen_US
dc.contributor.authorKritsadaporn Pornintaen_US
dc.contributor.authorSumeth Sommaneeen_US
dc.contributor.authorChatchadaporn Mahakunthaen_US
dc.contributor.authorThanongsak Chaiyasoen_US
dc.contributor.authorPhisit Seesuriyachanen_US
dc.contributor.authorAlissara Reungsangen_US
dc.contributor.authorNgoc Thao Ngan Trinhen_US
dc.contributor.authorSutee Wangtueaien_US
dc.contributor.authorSarana Rose Sommanoen_US
dc.contributor.authorNoppol Leksawasdien_US
dc.description.abstractThe (R)-phenylacetylcarbinol (PAC) batch biotransformation kinetics for partially purified Candida tropicalis TISTR 5350 pyruvate decarboxylase (PDC) were determined to validate a comprehensive mathematical model in 250 mL scale with 250 mM phosphate buffer/pH 7.0. PDC could convert initial 100/120 mM benzaldehyde/pyruvate substrates to the statistical significantly highest (p ≤ 0.05) maximum PAC concentration (95.8 ± 0.1 mM) and production rate (0.639 ± 0.001 mM min−1). A parameter search strategy aimed at minimizing overall residual sum of square (RSST) based on a system of six ordinary differential equations was applied to PAC biotransformation profiles with initial benzaldehyde/pyruvate concentration of 100/120 and 30/36 mM. Ten important biotransformation kinetic parameters were then elucidated including the zeroth order activation rate constant due to phosphate buffer species (ka) of (9.38 ± < 0.01) × 10–6% relative PDC activity min−1 mM−1. The validation of this model to independent biotransformation kinetics with initial benzaldehyde/pyruvate concentration of 50/60 mM resulted in relatively good fitting with RSST, mean sum of square error (MSE), and coefficient of determination (R2) values of 662, 17.4, and 0.9863, respectively.en_US
dc.titleValidation of mathematical model with phosphate activation effect by batch (R)-phenylacetylcarbinol biotransformation process utilizing Candida tropicalis pyruvate decarboxylase in phosphate bufferen_US
article.title.sourcetitleScientific Reportsen_US
article.volume11en_US Rajabhat Universityen_US Lam University, Ho Chi Minh Cityen_US Kaen Universityen_US Mai Universityen_US of Scienceen_US
Appears in Collections:CMUL: Journal Articles

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