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dc.contributor.authorAussara Panyaen_US
dc.contributor.authorPucharee Songprakhonen_US
dc.contributor.authorSuthida Panwongen_US
dc.contributor.authorKanyaluck Jantakeeen_US
dc.contributor.authorThida Kaewkoden_US
dc.contributor.authorYingmanee Tragoolpuaen_US
dc.contributor.authorNunghathai Sawasdeeen_US
dc.contributor.authorVannajan Sanghiran Leeen_US
dc.contributor.authorPiyarat Nimmanpipugen_US
dc.contributor.authorPa Thai Yenchitsomanusen_US
dc.description.abstractDengue virus (DENV) infection causes mild to severe illness in humans that can lead to fatality in severe cases. Currently, no specific drug is available for the treatment of DENV infection. Thus, the development of an anti-DENV drug is urgently required. Cordycepin (3′-deoxyadenosine), which is a major bioactive compound in Cordyceps (ascomycete) fungus that has been used for centuries in Chinese traditional medicine, was reported to exhibit antiviral activity. However, the anti-DENV activity of cordycepin is unknown. We hypothesized that cordycepin exerts anti-DENV activity and that, as an adenosine derivative, it inhibits DENV replication. To test this hypothesis, we investigated the anti-DENV activity of cordycepin in DENV-infected Vero cells. Cordycepin treatment significantly decreased DENV protein at a half-maximal effective concentration (EC50) of 26.94 μM. Moreover, DENV RNA was dramatically decreased in cordycepin-treated Vero cells, indicating its effectiveness in inhibiting viral RNA replication. Via in silico molecular docking, the binding of cordycepin to DENV non-structural protein 5 (NS5), which is an important enzyme for RNA synthesis, at both the methyltransferase (MTase) and RNA-dependent RNA polymerase (RdRp) domains, was predicted. The results of this study demonstrate that cordycepin is able to inhibit DENV replication, which portends its potential as an anti-dengue therapy.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectPharmacology, Toxicology and Pharmaceuticsen_US
dc.titleCordycepin inhibits virus replication in dengue virus-infected vero cellsen_US
article.volume26en_US Hospitalen_US Malayaen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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