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dc.contributor.authorOrapin Insuanen_US
dc.contributor.authorPhornphimon Janchaien_US
dc.contributor.authorBenchaluk Thongchuaien_US
dc.contributor.authorRujirek Chaiwongsaen_US
dc.contributor.authorSupaporn Khamchunen_US
dc.contributor.authorSomphot Saoinen_US
dc.contributor.authorWimonrut Insuanen_US
dc.contributor.authorPeraphan Pothacharoenen_US
dc.contributor.authorWaraporn Apiwatanapiwaten_US
dc.contributor.authorAntika Boondaengen_US
dc.contributor.authorPilanee Vaithanomsaten_US
dc.description.abstractBromelain is a mixture of proteolytic enzymes derived from pineapple (Ananas como-sus) fruit and stem possessing several beneficial properties, particularly anti-inflammatory activity. However, the molecular mechanisms underlying the anti-inflammatory effects of bromelain are unclear. This study investigated the anti-inflammatory effects and inhibitory molecular mechanisms of crude and purified rhizome bromelains on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophage cells. RAW264.7 cells were pre-treated with various concentrations of crude bromelain (CB) or purified bromelain (PB), and then treated with LPS. The production levels of pro-inflammatory cytokines and mediators, including nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α were determined by Griess and ELISA assays. The expressions of inducible nitric oxide synthetase (iNOS), cyclooxygenase (COX)-2, nuclear factor kappa B (NF-κB), and mitogen-activated protein kinases (MAPKs)-signaling pathway-related proteins were examined by western blot analysis. The pre-treatment of bromelain dose-dependently reduced LPS-induced pro-inflammatory cytokines and mediators, which correlated with downregulation of iNOS and COX-2 expressions. The inhibitory potency of PB was stronger than that of CB. PB also suppressed phosphorylated NF-κB (p65), nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha, extracellular signal-regulated kinases, c-Jun amino-terminal kinases, and p38 proteins in LPS-treated cells. PB then exhibited potent anti-inflammatory effects on LPS-induced inflammatory responses in RAW264.7 cells by inhibiting the NF-κB and MAPKs-signaling pathways.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleAnti-inflammatory effect of pineapple rhizome bromelain through downregulation of the NF-κB-and MAPKs-signaling pathways in lipopolysaccharide (LPS)-stimulated RAW264.7 cellsen_US
article.title.sourcetitleCurrent Issues in Molecular Biologyen_US
article.volume43en_US of Phayaoen_US Universityen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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