Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/75508
Title: RNA Interference Suppression of v-ATPase B and Juvenile Hormone Binding Protein Genes Through Topically Applied dsRNA on Tomato Leaves: Developing Biopesticides to Control the South American Pinworm, Tuta absoluta (Lepidoptera: Gelechiidae)
Authors: Govindaraju Ramkumar
Ramasamy Asokan
N. R. Prasannakumar
B. Kariyanna
Sengodan Karthi
Mona S. Alwahibi
Mohamed Soliman Elshikh
Ahmed Abdel-Megeed
Aml Ghaith
Sengottayan Senthil-Nathan
Kandaswamy Kalaivani
Wayne Brian Hunter
Patcharin Krutmuang
Authors: Govindaraju Ramkumar
Ramasamy Asokan
N. R. Prasannakumar
B. Kariyanna
Sengodan Karthi
Mona S. Alwahibi
Mohamed Soliman Elshikh
Ahmed Abdel-Megeed
Aml Ghaith
Sengottayan Senthil-Nathan
Kandaswamy Kalaivani
Wayne Brian Hunter
Patcharin Krutmuang
Keywords: Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 18-Nov-2021
Abstract: The South American pinworm Tuta absoluta (Meyrick) (Family: Gelechiidae) is one of the most devastating lepidopteran pests in the developing countries of South America, Africa, and Asia. This pest is classified as the most serious threat for tomato production worldwide. In the present study, we analyzed RNAi-mediated control through exogenously applied dsRNA delivery on tomato. The dsRNA treatments were made to target the juvenile hormone binding protein and the v-ATPase B. Both mRNA targets were cloned, validated by sequencing, and used to produce each dsRNA. After treatments the relative transcript expression was analyzed using qRTPCR to assess to efficacy of RNAi. A leaf-dip assay was used to provide late 2nd instar larvae three feeding access periods: 24, 48, and 72 h, to evaluate the effect of gene silencing of each target. Larvae were fed tomato leaves coated with five different RNAi concentrations (10, 20, 30, 40, and 50 micrograms/centimeter-squared), that suppressed two genes (juvenile hormone protein, JHBP, and vacuolar-type adenosine triphosphatase enzyme, v-ATPase). Treatments with dsRNA showed a significant increase in mortality at 24, 48, and 72 h after ingestion (P < 0.01, α = 0.05), along with reduced leaf damage, and increased feeding deterrence. The results suggest that these two RNAi products may provide a suitable treatment for control of this and other lepidopteran pests.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85120567817&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/75508
ISSN: 1664042X
Appears in Collections:CMUL: Journal Articles

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