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dc.contributor.authorSucheewin Krobthongen_US
dc.contributor.authorYodying Yingchutrakulen_US
dc.contributor.authorWonnop Visessanguanen_US
dc.contributor.authorThanisorn Mahatnirunkulen_US
dc.contributor.authorPawitrabhorn Samutrtaien_US
dc.contributor.authorChartchai Chaichanaen_US
dc.contributor.authorPhakorn Papanen_US
dc.contributor.authorKiattawee Choowongkomonen_US
dc.description.abstractExcessive lipid accumulation is a serious condition. Therefore, we aimed at developing safe strategies using natural hypolipidemic products. Lingzhi is an edible fungus and potential lipid suppression stimulant. To use Lingzhi as a functional hyperlipidemic ingredient, response surface methodology (RSM) was conducted to optimize the time (X1) and enzyme usage (X2) for the hydrolysate preparation with the highest degree of hydrolysis (DH) and % yield. We encapsulated the hydrolysates using nanoscale liposomes and used proteomics to study how these nano-liposomal hydrolysates could affect lipid accumulation in adipocyte cells. RSM analysis revealed X1 at 8.63 h and X2 at 0.93% provided the highest values of DH and % yields were 33.99% and 5.70%. The hydrolysates were loaded into liposome particles that were monodispersed. The loaded nanoliposomal particles did not significantly affect cell survival rates. The triglyceride (TG) breakdown in adipocytes showed a higher TG increase compared to the control. Lipid staining level upon the liposome treatment was lower than that of the control. Proteomics revealed 3425 proteins affected by the liposome treatment, the main proteins being TSSK5, SMU1, GRM7, and KLC4, associated with various biological functions besides lipolysis. The nano-liposomal Linzghi hydrolysate might serve as novel functional ingredients in the treatment and prevention of obesity.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectHealth Professionsen_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectSocial Sciencesen_US
dc.titleStudy of the lipolysis effect of nanoliposome-encapsulated Ganoderma lucidum protein hydrolysates on adipocyte cells using proteomics approachen_US
article.volume10en_US Hospitalen_US Universityen_US National Nanotechnology Centeren_US National Center for Genetic Engineering and Biotechnologyen_US National Science and Technology Development Agencyen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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