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dc.contributor.authorPrapas Patchaneeen_US
dc.contributor.authorNipa Chokesajjawateeen_US
dc.contributor.authorPannita Santiyanonten_US
dc.contributor.authorPhongsakorn Chuammitrien_US
dc.contributor.authorManu Deeudomen_US
dc.contributor.authorWilliam Monteithen_US
dc.contributor.authorSamuel K. Shepparden_US
dc.contributor.authorBen Pascoeen_US
dc.contributor.authorTeerarat Prasertseeen_US
dc.description.abstractSalmonella spp. is an important foodborne pathogen associated with consumption of contaminated food, especially food of livestock origin. Antimicrobial resistance (AMR) in Salmonella has been reported globally and increasing AMR in food production is a major public health issue worldwide. The objective of this study was to describe the genetic relatedness among Salmonella enterica isolates, which displayed identical DNA fingerprint profiles. Ten S. enterica isolates were selected from meat and human cases with an identical rep-PCR profile of serovars Rissen (n = 4), Weltevreden (n = 4), and Stanley (n = 2). We used long-read whole genome sequencing (WGS) on the MinION sequencing platform to type isolates and investigate in silico the presence of specific AMR genes. Antimicrobial susceptibility testing was tested by disk diffusion and gradient diffusion method to corroborate the AMR phenotype. Multidrug resistance and resistance to more than one antimicrobial agent were observed in eight and nine isolates, respectively. Resistance to colistin with an accompanying mcr-1 gene was observed among the Salmonella isolates. The analysis of core genome and whole genome MLST revealed that the Salmonella from meat and human salmonellosis were genetically related. Hence, it could be concluded that meat is one of the important sources for Salmonella infection in human.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleCharacterisation of Salmonella enterica clones carrying mcr-1 plasmids in meat products and patients in Northern Thailand using long read sequencingen_US
article.title.sourcetitleInternational Journal of Food Microbiologyen_US
article.volume358en_US of Bathen_US National Center for Genetic Engineering and Biotechnologyen_US of Songkla Universityen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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