Innovative biorefinery concept for utilizing uncooked rice berry wastewater on the growth of strawberry

Abstract

Annual fertilizer demand can be affected by population, economics, and food prices. Organic fertilizer lasts longer than mineral fertilizer. Plants prefer easily absorbed liquid nutrients. Uncooked rice berry water was utilized in this study according to the innovative biorefinery concept. The effects of sodium hypochlorite (NaOCl) in MS medium at five different concentrations (0, 0.5, 1, 2, and 3%) for 7 days without using an autoclave were measured; the results showed that MS medium with 2.3% NaOCl had the highest sterilization rate. The strawberry cultivar Praratchatan No. 80 seeds were then cultivated on a NaOCl-MS medium. The findings indicate MS medium enhanced productivity by 1%. The most shoots (8.56±0.34 shoots/explant) were produced by NaOCl, while MS media produced the most roots (5.55±0.78 roots/explant). The strawberry cultivar seeds were also cultivated on NaOCl-MS medium supplemented with uncooked rice berry wastewater at five different concentrations (0, 5, 10, 15, and 20%), as well as a combination of BA at 2 mg/L and NAA 0.1 mg/L. The results showed that MS medium supplements containing 10% uncooked rice berry wastewater + 2 mg/L BA + 0.1 mg/L NAA produced the most shoots (13.21±0.32 shoots/explant) and 2% NaOCl-MS medium + 10% uncooked rice berry wastewater produced the most roots (18.07±0.20 shoots/explant). This study found that MS medium supplemented with uncooked rice berry wastewater gave the best results regarding the growth in shoot and root production.