Bioremediation competence of Aspergillus flavus DDN on pond water contaminated by mining activities

Abstract

This research was performed to evaluate the possibilities of reducing the physicochemical properties of polluted pond water situated around the magnesite mine tailing through indigenous metal tolerant fungi. The physicochemical analysis results revealed that most of the physicochemical properties of pond water sample were crossing the permissible limits. From the muddy pond soil sample, Aspergillus flavus DDN was identified (through molecular characterization) as predominant metal tolerant fungal strain and it showed resistance to Cr(VI), Pb(II), Zn(II), Cd(II), and Mg(IV) up to 1000 μg mL−1 concentrations. This strain also effectively reduced (through biosorption) these metals in a short duration of the bioremediation process. In a lab-scale bioremediation study, the A. flavus DDN significantly reduced most of the physicochemical parameters crossing the permissible limit in polluted pond water in the presence of FM1 minimal media in 10 days of incubation. The dissolved oxygen level was significantly increased up to 74.91% from 5.86 ± 0.39 to 10.25 ± 0.95 in 10 days of treatment. The metal reduction and other physicochemical properties reduction were directly related to the biomass of A. flavus DDN. These findings suggest that A. flavus DDN can remove pollutants from magnesite mine tailing polluted pond water because elevated fungal biomass resulted in the highest percentage of pollutant reduction from the sample.