Nanocellulose production from rice straw derived-cellulose by enzymatic hydrolysis and its effect on lipase activity

Abstract

Rice straw derived-cellulose was applied as a potential source containing 92.77±0.71% (w/w) of cellulose for nanocellulose production using enzymatic hydrolysis. The nanocellulose content was found to be 28.28±0.38% (w/w), the cellulose residue remaining was 19.93±0.69%, while the efficiency of cellulase hydrolysis was approximately 80%. The rice straw nanocellulose was granular-shaped with a particle size range of 295-396 nm and gave a highly stable suspension due to a high zeta potential value of-45.8±0.4 mV. Furthermore, the effect of the granular nanocellulose on lipase activity was investigated on various substrates. The results showed that the granular nanocellulose significantly reduced lipase activity with a relative lipase activity of 90.51±1.39% and 74.32±0.96% of total activity using p-nitrophenyl laurate and olive oil as substrates, respectively. This research has shown that granular nanocellulose can be successfully produced from rice straw-derived cellulose by enzymatic hydrolysis under controlled reaction conditions. Moreover, the granular nanocellulose exhibited attractive physicochemical properties for the composite of nanocellulose with other materials to obtain novel property products, especially for dietary supplements and food ingredients in fat food applications.