Extended spectrum beta- lactamase producing escherichia coli from pork in Muang District, Chiang Mai Province

Abstract

Extended-spectrum beta-lactamase (ESBL)-producing E. coli that resist to beta-lactam antibiotics, have been widely reported and detected in food from many countries. The contaminated pork is one of the transmission routes with this bacterium to consumers. This study informed the proportion of ESBL-producing E. coli, antibiotic resistance pattern and ESBL-producing E. coli genes from pork in Muang District, Chiang Mai Province, Thailand which traditional spicy minced raw pork salad is a famous dish. The 100 samples were collected from 3 market types including fresh markets, pork retail stores, and supermarkets. After enrichment and inoculation on selective media, ESBL- producing E. coli were identified with double-disk diffusion method according to CLSI 2016. Antibiotic susceptibility was tested by Vitek 2 System and ESBL-encoding genes were determined by multiplex PCR. The results showed 69% of samples were positive to ESBL-producing E. coli including from fresh markets 97.22%, pork stores 80.95%, and supermarkets 39.53%. The most resistant were Ampicillin (100%), Piperacillin (100%), Cefalexin (100%), Cefpodoxime (100%), Cefovecin (100%), Ceftiofur (100%) follow by Gentamycin (89.86%), and Tetracycline (84.06%). All isolates were multiple drug resistance at least 6 antibiotics, AM, PIP, CN, CPD, CFO, CFT, GM, TE, C, SXT was the most common antimicrobial resistance pattern. Coexistence resistance of beta-lactams, aminoglycosides, and tetracyclines were observed. All susceptibility were Imipenem 100% and Amikacin 100%. All positive were CTX-M group follow by TEM (98.55%), SHV (1.45 %), and no OXA gene. This study showed high ESBL- producing E. coli proportion in pork might been the risk transmission route to customer though contaminated food chain. The reasonable antimicrobial using and choosing pork form reliable sources should be realized.