การเตรียม การหาลักษณะเฉพาะ และการทดสอบสมบัติของไฮโดรเจล เอ็น-ซักซินิลไคโตซาน-กราฟต์- พอลิ(เอ็น-ไอโซพรอพิลอะคริลาไมด์)/พอลิ(ไวนิลแอลกอฮอล์) สำหรับใช้เป็นวัสดุปิดแผล

Abstract

An ideal wound dressing should produce a suitably moist environment for healing of wound, absorb the surplus exudation, allow a gaseous swapping and easily to unwrap them without trauma. In addition, it has to be antimicrobial, nontoxic and biocompatible. One of the most interesting candidates used for wound dressing is a hydrogel. The objective of this research is to synthesize characterize hydrogel for use as wound dressing based on poly(N-isopropylacrylamide) (NIPAm), Nsuccinyl chitosan (NSC) and poly(vinyl alcohol) (PVA). Initially, chitosan was modified to NSC at the N-terminal of the glucosamine units and it was confirmed the success of the modification by Fourier transform infrared spectroscopy. NSC was then copolymerized with NIPAm and PVA in an aqueous solution at various w/w NSC:NIPAm ratio of 1:2:8, 1:4:8 and 1:6:8 using glutaraldehyde as a crosslinking agent. The hydrogels were synthesized via free radical copolymerization and characterization was carried out with Fourier transform infrared spectroscopy in transmission mode, differential scanning calorimetry, gel fraction, swelling ratio (SR), equilibrium water content (%EWC), water vapor transmission rate (%WVTR) and %water retention (%WR). Their morphology was investigated by scanning electron microscopy. The mechanical properties of the synthesized hydrogels were evaluated by tensile test. The results show that suitable gel properties and mechanical properties were obtained in the NSC:NIPAm ratio of 1:4 sample with the tensile stress (0.596 ± 0.015 MPa), Young’s modulus (0.042 ± 0.001 MPa), %EWC (92.56 ± 0.71%), %WVTR (78.87 ± 4.63%), SR (12.96 ± 1.34) and %WR (7.27 ± 0.82%), respectively. The images of scanning electron microscopy showed that the morphology of the hydrogels was porous. From tensile testing results, it clearly indicated that NIPAm can enhance the mechanical property of the hydrogel. From the above results, it can be concluded that the synthesized hydrogels in this research show the performance that close to the requirements of an ideal wound dressing.