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Title: In vitro Antioxidant Activity of Vitex negundo L. Leaf Extracts
Authors: Raghavendra H. Lakshmanashetty
Vijayananda B. Nagaraj
Madhumathi G. Hiremath
Vadlapudi Kumar
Keywords: Vitex negundo L
Reducing power
Antioxidant capacity
Issue Date: 2010
Publisher: Faculty of Science, Chiang Mai University
Citation: Chiang Mai Journal of Science 36, 3 (September 2010), 489-497
Abstract: In the present study, Vitex negundo L. leaf extracts were screened for their phytochemical composition and antioxidant activity. The yield of phytochemicals is in the order of ethanol extract > methanol extract > benzene extract > chloroform extract > hexane extract > ethyl acetate extract > petroleum extract. Finally we have selected ethanol extract for the antioxidant activity. Total phenolic content was estimated as gallic acid equivalents (249.96 +/- 8.34 mg/g of extract) and total flavonoids estimated as catechin equivalents (166.67 +/- 9.14 mg/g of extract). Free radical scavenging activity was determined by DPPH assay (79.82 +/- 2.99% at 500 μg/ml concentration). Total antioxidant capacity of the extract (214.81 +/- 4.07 mg ascorbic acid equivalent/g) was determined. Ferrous chelating ability was found to be 84.15 +/- 1.65% at 500 μg/ml concentration and reductive ability of the extract was found to be 0.918 0.035 absorbance unit at 600 μg/ml concentration. The antioxidant activity of Vitex negundo L. is mainly due to the presence of the phenolic compounds like, flavonoids and flavonols.
Description: The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.
ISSN: 2465-3845
Appears in Collections:CMUL: Journal Articles

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