Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/71923
Title: Quercetin enhances and modulates the fungal killing efficacy of chicken heterophils through immunological recognition, effector functions, and resolution
Authors: Antika Boonlaos
Wiriyachayon Wechsirisan
Pawitree Chaibuth
Vena Chupia
Suwit Chotinun
Phongsakorn Chuammitri
Keywords: Immunology and Microbiology
Medicine
Veterinary
Issue Date: 1-Feb-2021
Abstract: © 2020 Elsevier Ltd Herbal compound, quercetin, has previously been shown its modulatory effects on mammalian neutrophils and avian counterpart. However, at this instance it is not clear how quercetin promotes its effects on fungal and yeast killing in chicken heterophils. In the present study, we have proved that quercetin exerts the significant modulatory effects against pathogenic yeast (Candida albicans) in freshly isolated heterophils from Thai native broiler chicken. This substance is shown to facilitate heterophil effector functions through the reduction of ROS generation, and promotion of phagocytosis and candidacidal killing. The quercetin effects on zymosan recognition and migration of cells toward zymosan are subtle, but insignificant differed from control, whereas cell migration towards live Candida is markedly differed. We also find the abundant release of heterophil extracellular traps (HETs) from quercetin-primed cells. From a gene expression standpoint, cells received quercetin display the up-regulation of fungal recognition and migratory genes. The quercetin shows anti-inflammatory function by suppression of pro-inflammatory cytokine genes as well as most of ROS-related genes. Collectively, our findings highlight and provide clues for a promising utilization of quercetin in chicken innate immunity to further combat the fungal infections.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85097096117&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/71923
ISSN: 18781667
01479571
Appears in Collections:CMUL: Journal Articles

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