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dc.contributor.authorAnurak Wongtaen_US
dc.contributor.authorSurat Hongsibsongen_US
dc.contributor.authorSomporn Chantaraen_US
dc.contributor.authorMookda Pattarawarapanen_US
dc.contributor.authorRatana Sapbamreren_US
dc.contributor.authorKorawan Sringarmen_US
dc.contributor.authorZhen Lin Xuen_US
dc.contributor.authorHong Wangen_US
dc.date.accessioned2021-01-27T03:52:23Z-
dc.date.available2021-01-27T03:52:23Z-
dc.date.issued2020-01-01en_US
dc.identifier.issn23147156en_US
dc.identifier.issn23148861en_US
dc.identifier.other2-s2.0-85099314047en_US
dc.identifier.other10.1155/2020/8821181en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85099314047&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/71540-
dc.description.abstract© 2020 Anurak Wongta et al. Amyloid beta peptides (Aβ1-42) have been found to be associated with the cause of Alzheimer's disease (AD) and dementia. Currently, methods for detecting Aβ1-42 are complicated and expensive. The present study is aimed at developing an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) to detect Aβ1-42 by using a polyclonal antibody from alpaca, an application used in urine samples. The serum was collected from the alpaca after immunizing it with Aβ1-42 at 500 μg/injection 5 times. The ic-ELISA was developed and showed a half-maximal inhibitory concentration (IC50) of 103.20 ng/ml. The limit of detection (LOD) was 0.39 ng/100 μl. The cross-reactivity was tested with Aβ1-40 and 8 synthesized peptides that had sequence similarities to parts of Aβ1-42. The cross-reactivity of Aβ1-40 and peptide 1 (DAEFRHDSGYE) was 55% and 69.4%, respectively. The ic-ELISA was applied to analyze Aβ1-42 in the urine and precipitated protein urine samples. This method can be used for detecting a normal level of total soluble Aβ (approximately 1 ng in 5 mg of precipitated urine protein) and can be used for detecting the early stages of AD. It is considered to be an easy and inexpensive method for monitoring and diagnosing AD.en_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleDevelopment of an Immunoassay for the Detection of Amyloid Beta 1-42 and Its Application in Urine Samplesen_US
dc.typeJournalen_US
article.title.sourcetitleJournal of Immunology Researchen_US
article.volume2020en_US
article.stream.affiliationsSouth China Agricultural Universityen_US
article.stream.affiliationsChiang Mai Universityen_US
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